6R16

Crystal structure of the SUN1-KASH4 6:6 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	104.370, 117.210, 138.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.830 - 2.750
R-factor	0.2208
Rwork	0.219
R-free	0.25490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6r15
RMSD bond length	0.002
RMSD bond angle	0.445
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.830	2.850
High resolution limit [Å]	2.750	2.750
Rmerge	0.095	1.124
Rmeas	0.111	1.355
Rpim	0.056	0.741
Number of reflections	44728	4500
<I/σ(I)>	15.4	1.4
Completeness [%]	99.7	97.5
Redundancy	7.1	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.06M MgCl2; 0.06M CaCl2; 0.1M Imidazole pH 6.5; 0.1M MES (acid) pH 6.5 + 18% Ethylene glycol; 18% PEG 8K