6R15

Crystal structure of the SUN1-KASH1 6:6 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9282
Spacegroup name	P 63 2 2
Unit cell lengths	80.450, 80.450, 182.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	65.090 - 1.820
R-factor	0.1598
Rwork	0.159
R-free	0.18170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dxr
RMSD bond length	0.013
RMSD bond angle	0.995
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.090	1.850
High resolution limit [Å]	1.820	1.820
Rmerge	0.083	2.141
Rmeas	0.085	2.192
Rpim	0.019	0.465
Number of reflections	32282	2344
<I/σ(I)>	21.5	1.7
Completeness [%]	100.0
Redundancy	20.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.09M NaF; 0.09M NaBr; 0.09M NaI; 0.1M Sodium HEPES pH 7.5; 0.1M MOPS (acid) pH 7.5; 18% PEGMME 550; 18% PEG 20K