6QZV
DPP9 bound to a dipeptide (MP) from the N-terminus of BRCA2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.00002 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 119.516, 117.219, 163.401 |
| Unit cell angles | 90.00, 105.72, 90.00 |
Refinement procedure
| Resolution | 43.470 - 3.000 |
| R-factor | 0.28871 |
| Rwork | 0.286 |
| R-free | 0.34517 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6eoq |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.470 | 3.190 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.130 | 0.300 |
| Number of reflections | 76534 | 13092 |
| <I/σ(I)> | 5.9 | 3 |
| Completeness [%] | 87.8 | 88.8 |
| Redundancy | 2.65 | |
| CC(1/2) | 0.980 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 293 | 20 mg/ml of protein, 10% PEG 8000, 25% glycerol, 0.16 M calcium acetate, 0.08 M cacodilate pH 6.25. Drops are set in a 1:1 ratio. |
