6QZO
Crystal structure of DyP-type peroxidase from Cellulomonas bogoriensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-08-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 62 |
| Unit cell lengths | 173.989, 173.989, 283.003 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.870 - 2.400 |
| R-factor | 0.24334 |
| Rwork | 0.242 |
| R-free | 0.26718 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jxu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.476 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.900 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rpim | 0.119 | 0.569 |
| Number of reflections | 184630 | 9000 |
| <I/σ(I)> | 7 | 1.5 |
| Completeness [%] | 97.9 | 96.2 |
| Redundancy | 9.9 | 9.8 |
| CC(1/2) | 0.985 | 0.463 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 20% PEG300, 0.1M Tris pH 8.5, 5% PEG8000 and 10% glycerol |
