6QZJ

Crystal structure of human Aquaporin 7 at 2.2 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	I 4
Unit cell lengths	86.761, 86.761, 83.544
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.772 - 2.200
R-factor	0.2105
Rwork	0.209
R-free	0.24320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f7h
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.350	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.027	0.673
Rmeas	0.032	0.797
Rpim	0.016	0.418
Number of reflections	15584	1364
<I/σ(I)>	17.2
Completeness [%]	98.8	99.6
Redundancy	3.3	3.4
CC(1/2)	1.000	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	85 mM Tris pH 8.0, 37 % v/v PEG 200 and 0.80 % v/v OG