Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.420, 76.730, 90.130 |
| Unit cell angles | 90.00, 93.50, 90.00 |
Refinement procedure
| Resolution | 55.537 - 2.660 |
| Rwork | 0.200 |
| R-free | 0.25390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dlh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.684 |
| Data reduction software | xia2 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.540 | 2.730 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.196 | 1.043 |
| Rmeas | 0.229 | 1.215 |
| Rpim | 0.116 | 0.616 |
| Number of reflections | 26304 | 1929 |
| <I/σ(I)> | 6.3 | 1.5 |
| Completeness [%] | 99.1 | 98.7 |
| Redundancy | 3.7 | |
| CC(1/2) | 0.466 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 0.1 M Tris 0.2 M NH4 SO4 25% PEG 4000 |
