6QYI

Structure of HPAB from E.coli in complex with FAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97625
Spacegroup name	P 65 2 2
Unit cell lengths	188.160, 188.160, 162.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.090 - 1.800
R-factor	0.165
Rwork	0.165
R-free	0.17200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.930
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	115.064	1.832
High resolution limit [Å]	1.800	1.801
Rmerge	0.164	3.926
Rmeas	0.170	4.065
Rpim	0.044	1.048
Number of reflections	155091	7637
<I/σ(I)>	11.554	0.773
Completeness [%]	100.0	100
Redundancy	14.78	14.6
CC(1/2)	0.999	0.348

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	HEPES 0.1M pH 7.5 PEG 400 30% (w/v) CaCl2 0.2 M MgCl2 0.1-0.2M