6QY8
Human CSNK2A2 bound to ERB-041
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-13 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.719, 114.139, 133.750 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 86.800 - 1.700 |
| R-factor | 0.26459 |
| Rwork | 0.263 |
| R-free | 0.29116 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ofm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.372 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.820 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.210 | 0.320 |
| Number of reflections | 153463 | 7634 |
| <I/σ(I)> | 3.8 | 2.1 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% (w/v) PEG10000, 0.1M HEPES pH 7.5 |
