6QWP
Crystal structure of T7 bacteriophage portal protein, 13mer, closed valve
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0679 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 261.570, 261.570, 256.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.560 - 3.400 |
| R-factor | 0.23203 |
| Rwork | 0.230 |
| R-free | 0.27534 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.196 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.560 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.025 | |
| Number of reflections | 110605 | |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 95.7 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.969 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15 % tacsimate, 0.1 M HEPES pH 7.0, 12 % (w/v) PEG 3350 |
