6QVZ

The Transcriptional Regulator PrfA-L140H mutant from Listeria Monocytogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9677
Spacegroup name	P 21 21 21
Unit cell lengths	47.821, 88.465, 116.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.216 - 2.705
R-factor	0.1913
Rwork	0.188
R-free	0.25390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f1r
RMSD bond length	0.002
RMSD bond angle	0.442
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.146	0.715
Rpim	0.094	0.460
Number of reflections	13826	1733
<I/σ(I)>	8.4	2
Completeness [%]	98.5	94.7
Redundancy	6.2	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	Droplets of 2 to 4 microl protein solution in 50 mM sodium phosphate pH 6.5 and 200 mM NaCl at 3 mg per ml were mixed with 2 microl reservoir solution consisting of 22-25% PEG 4000, 100 mM sodium citrate pH 5.5, and 17% isopropanol.