6QV5
Crystal structure of the CHAD domain from the plant Ricinus communis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-31 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.999859 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 116.427, 116.427, 119.248 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.650 - 2.300 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.24200 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.890 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.650 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.172 | 3.677 |
| Number of reflections | 40129 | 6493 |
| <I/σ(I)> | 15.5 | 1 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 21.2 | 21.5 |
| CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 3 M NaCl, 0.1 M Bis-Tris pH 6.0 |
