6QV4

Crystal structure of the Ski2 RNA-helicase Brr2 from Chaetomium thermophilum bound to ATP-gamma-S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.8814
Spacegroup name	P 31
Unit cell lengths	124.818, 124.818, 127.087
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.526 - 2.800
R-factor	0.2112
Rwork	0.209
R-free	0.26020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5m59
RMSD bond length	0.002
RMSD bond angle	0.522
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.860
High resolution limit [Å]	2.700	2.700
Rmeas	0.151	2.516
Number of reflections	60260	45316
<I/σ(I)>	10.19
Completeness [%]	98.9
Redundancy	10.4	10.3
CC(1/2)	0.999	0.387

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Tris-HCl, Peg8000, calcium acetate