6QV3

Crystal structure of the Ski2 RNA-helicase Brr2 from Chaetomium thermophilum bound to ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9184
Spacegroup name	P 31
Unit cell lengths	125.174, 125.174, 127.445
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.652 - 2.900
R-factor	0.2228
Rwork	0.220
R-free	0.27090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5m59
RMSD bond length	0.003
RMSD bond angle	0.608
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.970
High resolution limit [Å]	2.796	2.796
Rmeas	0.157	1.731
Number of reflections	54974
<I/σ(I)>	5.19
Completeness [%]	99.5
Redundancy	8.2
CC(1/2)	0.990	0.201

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	Tris-HCl, PEG 8000, calcium acetate