6QUK
Protein crystallization by ionic liquid hydrogel support: glucose isomerase grown by using ionic liquid hydrogel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97779 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 85.900, 92.598, 99.111 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.980 - 1.580 |
R-factor | 0.16824 |
Rwork | 0.167 |
R-free | 0.19449 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oad |
RMSD bond length | 0.008 |
RMSD bond angle | 0.635 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | Sir2014 |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.900 | 1.620 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.099 | 0.664 |
Rmeas | 0.115 | 0.791 |
Rpim | 0.070 | 0.492 |
Number of reflections | 103356 | 7748 |
<I/σ(I)> | 11.1 | 3.4 |
Completeness [%] | 95.6 | 97.4 |
Redundancy | 4.2 | 4.1 |
CC(1/2) | 0.995 | 0.622 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 298 | protein 26mg/m in HEPES 10mM pH 7 and MgCl2 1mM Reservoir: (NH4)2SO4 1.5M |