6QTA

Crystal structure of Rea1-MIDAS/Rsa4-UBL complex from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-05
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.87290
Spacegroup name	P 43 21 2
Unit cell lengths	115.675, 115.675, 74.623
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.580 - 1.890
R-factor	0.1776
Rwork	0.176
R-free	0.21140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6QT8 and 4WJS (UBL domain only)
RMSD bond length	0.018
RMSD bond angle	1.412
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.580	1.950
High resolution limit [Å]	1.890	1.890
Number of reflections	41048
<I/σ(I)>	12.86
Completeness [%]	100.0
Redundancy	13.2
CC(1/2)	0.999	0.478

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	2 M (NH4)2SO4, 5% PEG 400 and 0.1 M MES at pH 6.5