6QQF

Room temperature structure of Hen Egg White Lysozyme recorded after an accumulated dose of 100 kGy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	293
Detector technology	PIXEL
Collection date	2018-08-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.07
Spacegroup name	P 43 21 2
Unit cell lengths	79.010, 79.010, 38.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.510 - 1.950
R-factor	0.15675
Rwork	0.152
R-free	0.22987
RMSD bond length	0.007
RMSD bond angle	1.426
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.510	2.000
High resolution limit [Å]	1.950	1.950
Number of reflections	9218	665
<I/σ(I)>	10.45	2.73
Completeness [%]	99.6	99.7
Redundancy	6.7	7.2
CC(1/2)	0.997	0.708

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8	293	6-9% NaCl, 0.1M Na Acetate pH4.8