6QQE

Room temperature structure of Hen Egg White Lysozyme recorded after an accumulated dose of 20 kGy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.07
Spacegroup name	P 43 21 2
Unit cell lengths	79.149, 79.149, 38.097
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.610 - 1.370
R-factor	0.15985
Rwork	0.158
R-free	0.18750
RMSD bond length	0.005
RMSD bond angle	1.342
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.620	1.410
High resolution limit [Å]	1.370	1.370
Number of reflections	25762	1828
<I/σ(I)>	17.1	2.24
Completeness [%]	98.9	96.9
Redundancy	7	7
CC(1/2)	0.999	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8	293	6-9% NaCl, 0.1M Na Acetate pH 4.8