6QQD

Cryogenic temperature structure of Hen Egg White Lysozyme recorded after an accumulated dose of 10 MGy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	P 43 21 2
Unit cell lengths	77.960, 77.960, 37.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.710 - 1.920
R-factor	0.15577
Rwork	0.152
R-free	0.21594
RMSD bond length	0.009
RMSD bond angle	1.572
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.710	1.970
High resolution limit [Å]	1.920	1.920
Number of reflections	8490	640
<I/σ(I)>	15.34	1.88
Completeness [%]	91.8	95.7
Redundancy	6.4	6.6
CC(1/2)	0.999	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8	293	6-9% NaCl, 0.1M Na Acetate pH 4.8