6QQB

Room temperature structure of the fluorescent protein Cerulean recorded after an accumulated dose of 147 kGy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	293
Detector technology	PIXEL
Collection date	2014-12-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	52.040, 62.670, 71.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.150 - 2.450
R-factor	0.18192
Rwork	0.177
R-free	0.26592
RMSD bond length	0.011
RMSD bond angle	1.570
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0218)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.150	2.510
High resolution limit [Å]	2.450	2.450
Number of reflections	9048	670
<I/σ(I)>	8.9	2.08
Completeness [%]	99.9	100
Redundancy	6.6	7.4
CC(1/2)	0.997	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	15% PEG 8000, 0.1M MGCL2, 0.1 M HEPES