6QPH
Dunaliella minimal PSI complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-07-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 158.881, 100.506, 191.040 |
| Unit cell angles | 90.00, 91.73, 90.00 |
Refinement procedure
| Resolution | 48.947 - 3.400 |
| R-factor | 0.3377 |
| Rwork | 0.337 |
| R-free | 0.35630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l8r |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.876 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.947 | 3.500 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rpim | 0.080 | 0.636 |
| Number of reflections | 83250 | 4575 |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | Sodium cacodylate 50mM, MgCl2 100mM, PEG1500 8-11% |
