6QNW
Influenza A Polymerase Heterotrimer Human H3N2 Northern Territory 1968
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.968620 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 336.480, 191.895, 235.735 |
| Unit cell angles | 90.00, 91.50, 90.00 |
Refinement procedure
| Resolution | 138.620 - 3.310 |
| R-factor | 0.2377 |
| Rwork | 0.235 |
| R-free | 0.27870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d9a |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.618 |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 235.654 | 3.916 |
| High resolution limit [Å] | 3.310 | 3.321 |
| Rmerge | 0.459 | 2.243 |
| Rmeas | 0.476 | 2.363 |
| Rpim | 0.126 | 0.626 |
| Number of reflections | 123159 | 9466 |
| <I/σ(I)> | 4.8 | 2 |
| Completeness [%] | 95.3 | 80.7 |
| Redundancy | 14.4 | 13.7 |
| CC(1/2) | 0.988 | 0.646 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 1-1.6 M K/Na phosphate |
