6QN7
Structure of bovine anti-RSV hybrid Fab B4HC-B13LC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96858 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.390, 86.030, 164.960 |
| Unit cell angles | 90.00, 98.62, 90.00 |
Refinement procedure
| Resolution | 45.958 - 2.150 |
| R-factor | 0.2041 |
| Rwork | 0.203 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.912 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_3386: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.100 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.094 | |
| Number of reflections | 61135 | 2316 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 95.7 | 72.4 |
| Redundancy | 6.1 | 3.7 |
| CC(1/2) | 1.000 | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20.0% w/v Polyethylene glycol 3350 0.2 M Ammonium Chloride |
