6QLZ

IDOL F3ab subdomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	56.365, 69.536, 74.318
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.343
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2he7
Data scaling software	SCALA (3.3.20)
Refinement software	BUSTER

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	74.318	74.318	2.470
High resolution limit [Å]	2.343	7.410	2.343
Rmerge		0.027	1.147
Rmeas	0.108	0.034	1.506
Rpim	0.050	0.016	0.711
Number of reflections	12704	466	1757
<I/σ(I)>	11	18.4	0.7
Completeness [%]	99.3	98.6	97.2
Redundancy	4.6	4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	100mM Hepes pH 7.0 10% (w/v) PEG 6000