6QL4
Crystal structure of nucleotide-free Mgm1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-10 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 147.400, 147.400, 344.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.133 - 3.600 |
R-factor | 0.244 |
Rwork | 0.243 |
R-free | 0.25230 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.976 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AutoSol |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.133 | 49.133 | 3.690 |
High resolution limit [Å] | 3.600 | 16.100 | 3.600 |
Rmerge | 0.034 | 1.839 | |
Rmeas | 0.038 | 1.992 | |
Number of reflections | 44814 | 896 | 6118 |
<I/σ(I)> | 7.96 | 36.74 | 1.07 |
Completeness [%] | 99.8 | 94.7 | 98.4 |
Redundancy | 6.9 | 5.999 | 6.761 |
CC(1/2) | 0.999 | 0.352 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 8% PEG400, 3% isopropanol, 100 mM Na-citrate buffer (pH 5.5) |