6QKV
Structure of YibK from P. aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 85.661, 167.114, 48.924 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.699 - 2.010 |
| R-factor | 0.1953 |
| Rwork | 0.192 |
| R-free | 0.23460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mxi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.969 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.700 | 2.130 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmeas | 0.071 | 0.665 |
| Number of reflections | 23919 | 3813 |
| <I/σ(I)> | 18.52 | 2.84 |
| Completeness [%] | 99.5 | 99.2 |
| Redundancy | 5.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.15 M ammonium sulfate, 0.9 M lithium sulfate, 0.1 M sodium citrate buffer, pH 5.6 |
