6QJD

Crystal Structure of the truncated form of the third PDZ domain of PSD-95: residues 302-392

Replaces:  5OHW

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96864
Spacegroup name	P 1 21 1
Unit cell lengths	54.118, 53.968, 62.613
Unit cell angles	90.00, 93.25, 90.00

Refinement procedure

Resolution	19.600 - 1.551
R-factor	0.2183
Rwork	0.216
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k82
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.600	19.600	1.580
High resolution limit [Å]	1.550	8.490	1.550
Rmerge	0.144	0.126	0.487
Number of reflections	48816	267	2452
<I/σ(I)>	5.4
Completeness [%]	94.1	80.9	96.7
Redundancy	3.4	4.3	3.1
CC(1/2)	0.983	0.980	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	298	0.2 M ammonium sulphate, 30% PEG 4000