6QJA
Organizational principles of the NuMA-Dynein interaction interface and implications for mitotic spindle functions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-26 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.730, 112.850, 135.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.900 - 1.540 |
| R-factor | 0.1717 |
| Rwork | 0.170 |
| R-free | 0.21470 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.670 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.910 | 1.570 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.036 | |
| Rmeas | 0.039 | |
| Rpim | 0.015 | |
| Number of reflections | 104874 | |
| <I/σ(I)> | 22.9 | 1.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.5 | |
| CC(1/2) | 1.000 | 0.539 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1 M Bis-Tris Propane, 0.2 M Magnesium Chloride, 28 % PEG 4000 |
