6QIE

Crystal structure of DEAH-box ATPase Prp43-S387G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97620
Spacegroup name	P 21 21 21
Unit cell lengths	88.640, 102.980, 118.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.848 - 2.700
R-factor	0.2299
Rwork	0.229
R-free	0.25370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ltj
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	77.848	77.848	2.800
High resolution limit [Å]	2.700	50.000	2.700
Rmerge	0.112	0.011	1.478
Rmeas	0.119	0.013	1.566
Number of reflections	30459	4	3066
<I/σ(I)>	14.78	64.61	1.64
Completeness [%]	99.8	57.1	99.8
Redundancy	9.115	2.75	9.244
CC(1/2)	0.999	1.000	0.648

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100mM HEPES pH7, 3% PEG4000, 35% MPD