6QH5

AP2 clathrin adaptor mu2T156-phosphorylated core in closed conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.920
Spacegroup name	P 31 2 1
Unit cell lengths	121.037, 121.037, 257.671
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.520 - 2.560
R-factor	0.22614
Rwork	0.225
R-free	0.25689
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vgl
RMSD bond length	0.015
RMSD bond angle	1.793
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.520	2.620
High resolution limit [Å]	2.560	2.560
Rmerge	0.320
Rmeas	0.339
Rpim	0.109
Number of reflections	71252
<I/σ(I)>	8.4
Completeness [%]	99.8
Redundancy	17.9
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	289	20% PEG 1000; 100 mM Na+/K+ phosphate buffer (pH 7.2), 200 mM NaCl, and 10 mM DTT