6QH1
The structure of Schizosaccharomyces pombe PCNA in complex with an Spd1 derived peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8729 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.860, 71.650, 84.020 |
| Unit cell angles | 90.00, 115.75, 90.00 |
Refinement procedure
| Resolution | 75.680 - 2.900 |
| R-factor | 0.2356 |
| Rwork | 0.234 |
| R-free | 0.26150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wgw |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.889 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.700 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rpim | 0.552 | |
| Number of reflections | 19183 | |
| <I/σ(I)> | 6.5 | 1.5 |
| Completeness [%] | 99.1 | 99.2 |
| Redundancy | 4.1 | 4.3 |
| CC(1/2) | 0.991 | 0.096 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | 100 mM TRIS-HCl pH 7.5, 18% (w/v) PEG4000 and 6% (v/v) glycerol, in the presence of 1.9 mM Spd1(27-46). [PCNA]=0.17 mM (5 mg/mL) |
