6QFL

Structure of the mitogen activated kinase kinase 7 active conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	62.000, 68.800, 85.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.058 - 2.200
R-factor	0.2169
Rwork	0.215
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dyl
RMSD bond length	0.001
RMSD bond angle	0.403
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.058	2.400
High resolution limit [Å]	2.200	2.200
Rmeas	0.075	0.447
Number of reflections	19157	3534
<I/σ(I)>	17.11	3.54
Completeness [%]	92.8	81.8
Redundancy	5.8	5.93
CC(1/2)	0.997	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2 M Sodiumcitrate 20 % PEG3350