6QEP
EngBF DARPin Fusion 4b H14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.99987 |
| Spacegroup name | P 65 |
| Unit cell lengths | 192.690, 192.690, 123.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.280 - 2.600 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.21500 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.280 | 2.693 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmeas | 0.172 | 3.263 |
| Number of reflections | 80365 | |
| <I/σ(I)> | 16.78 | |
| Completeness [%] | 99.9 | |
| Redundancy | 20.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.1 | 277.15 | MPD, PEG 20,000, sodium chloride, manganese chloride, MES |
