6QE0
Structure of E.coli RlmJ in complex with a bisubstrate analogue (BA2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-06 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98010 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.820, 58.400, 49.230 |
| Unit cell angles | 90.00, 97.32, 90.00 |
Refinement procedure
| Resolution | 37.460 - 1.394 |
| R-factor | 0.2265 |
| Rwork | 0.224 |
| R-free | 0.26640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4blv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.460 | 1.440 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.076 | 2.033 |
| Number of reflections | 53277 | |
| <I/σ(I)> | 12.71 | |
| Completeness [%] | 98.7 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M BIS-Tris pH 6.5, 25% (w/v) PEG3350 |
