6QDM
Molecular features of the UNC-45 chaperone critical for binding and folding muscle myosin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97917 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.392, 97.610, 148.882 |
| Unit cell angles | 90.00, 93.41, 90.00 |
Refinement procedure
| Resolution | 48.805 - 3.800 |
| R-factor | 0.2812 |
| Rwork | 0.280 |
| R-free | 0.30980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i2z |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.481 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 4.010 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmeas | 0.062 | 1.275 |
| Number of reflections | 22138 | |
| <I/σ(I)> | 9.1 | 1.1 |
| Completeness [%] | 98.9 | 96.2 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 100 mM Tris HCl pH 8.5 32 % glycerol ethoxylate |
