6QCW

Crystal structure of influenza B polymerase initiation state with capped 14-mer RNA primer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.977
Spacegroup name	P 32 2 1
Unit cell lengths	200.354, 200.354, 256.466
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.880
R-factor	0.21548
Rwork	0.215
R-free	0.23391
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5msg
RMSD bond length	0.003
RMSD bond angle	0.731
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.120
High resolution limit [Å]	2.880	2.880
Rmeas	0.185	1.500
Number of reflections	81417
<I/σ(I)>	8.2	1.8
Completeness [%]	60.7	60.9
Redundancy	8.2	10
CC(1/2)	0.995	0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	277	Influenza B polymerase at 9 mg per ml in was mixed with 40 microM vRNA 5 prime end 14-mer, 40 microM vRNA 3 prime end 21-mer and 80 microM 14-mer capped RNA. The mother liquor contained 200 mM di-ammonium phosphate and 100 mM sodium acetate between pH 4.0 and 4.4.