6QC0
PCNA complex with Cdt2 C-terminal PIP-box peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-12 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.872600 |
| Spacegroup name | P 61 |
| Unit cell lengths | 151.369, 151.369, 91.489 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.730 - 3.500 |
| R-factor | 0.19836 |
| Rwork | 0.196 |
| R-free | 0.25164 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.806 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.700 | 3.830 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 14850 | |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 97.1 | |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 10% (w/v) PEG 6000 100 mM MES/HCl |
