6QBW

Structure of the HTLV-2 integrase catalytic core domain in complex with calcium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	291
Detector technology	PIXEL
Collection date	2018-05-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 43 3 2
Unit cell lengths	112.082, 112.082, 112.082
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.120 - 2.400
Rwork	0.247
R-free	0.28190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qbt
Data reduction software	xia2
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.120	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.028	0.310
Number of reflections	9932	1872
<I/σ(I)>	13.8	1.8
Completeness [%]	100.0	100
Redundancy	1.8	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	100 mM Tris pH 8, 18% PEG 6000, 250 calcium chloride dihydrate