6QBT
Structure of the HTLV-2 integrase catalytic core domain in complex with magnesium (trimeric form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 291 |
Detector technology | PIXEL |
Collection date | 2017-10-02 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 115.920, 115.920, 115.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.980 - 2.290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cz1 |
Data reduction software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.980 | 2.370 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.023 | 0.290 |
Number of reflections | 12518 | 1194 |
<I/σ(I)> | 24.2 | 2.5 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 1.8 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 100 mM Tris pH 8.5, 14% PEG 8000, 100 mM magnesium chloride |