6QBT

Structure of the HTLV-2 integrase catalytic core domain in complex with magnesium (trimeric form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	291
Detector technology	PIXEL
Collection date	2017-10-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9762
Spacegroup name	P 43 3 2
Unit cell lengths	115.920, 115.920, 115.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.980 - 2.290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cz1
Data reduction software	xia2
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.980	2.370
High resolution limit [Å]	2.290	2.290
Rmerge	0.023	0.290
Number of reflections	12518	1194
<I/σ(I)>	24.2	2.5
Completeness [%]	99.9	99.9
Redundancy	1.8	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	100 mM Tris pH 8.5, 14% PEG 8000, 100 mM magnesium chloride