6QBF

Crystal structure of the pathological D187N variant of calcium-free human gelsolin.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9920
Spacegroup name	P 4 21 2
Unit cell lengths	169.673, 169.673, 151.089
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.880 - 3.499
R-factor	0.2211
Rwork	0.218
R-free	0.27590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ffn
RMSD bond length	0.002
RMSD bond angle	0.511
Data reduction software	DIALS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.980	3.690
High resolution limit [Å]	3.499	3.499
Rmeas	0.482	0.971
Number of reflections	28491
<I/σ(I)>	4.4	2
Completeness [%]	99.5	96.6
Redundancy	10.3	8.1
CC(1/2)	0.948	0.265

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	1.3 M Ammonium sulfate, 19% glycerol, 100 mM Tris HCl