6QBC
structure of anti-Mcl1 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97623 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 70.540, 70.540, 168.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.620 - 1.560 |
| R-factor | 0.205 |
| Rwork | 0.204 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.090 | 1.600 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Number of reflections | 70030 | |
| <I/σ(I)> | 19.1 | 1.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9.3 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.8 M (NH4)2SO4 12% EtOH 0.1 M PCPT 8.5 |
