6QB7
Structure of the H1 domain of human KCTD16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9282 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.427, 105.620, 107.554 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.000 - 2.230 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.27470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Unpublished |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.431 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.310 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Number of reflections | 42017 | |
| <I/σ(I)> | 9.22 | |
| Completeness [%] | 97.4 | |
| Redundancy | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Mother liquor: 0.8M ammonium phosphate monobasic -- 0.8M ammonium phosphate dibasic 1:1 drops with protein in: 300 mM NaCl, 50 mM HEPES pH 7.5, 1 mM TCEP, 5 mM DTT |
