6Q9Z
Crystal structure of the pathological G167R variant of calcium-free human gelsolin,
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-03 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 170.914, 170.914, 151.624 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.467 - 3.800 |
| R-factor | 0.2203 |
| Rwork | 0.216 |
| R-free | 0.26410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ffn |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.582 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.470 | 4.100 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmeas | 0.319 | |
| Number of reflections | 22724 | 4580 |
| <I/σ(I)> | 8.1 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.3 | 11.9 |
| CC(1/2) | 0.993 | 0.751 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.5 M ammonium sulfate, 20% glycerol, 100 mM Tris-HCl |
