6Q9Z

Crystal structure of the pathological G167R variant of calcium-free human gelsolin,

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-03
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.000
Spacegroup name	P 4 21 2
Unit cell lengths	170.914, 170.914, 151.624
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.467 - 3.800
R-factor	0.2203
Rwork	0.216
R-free	0.26410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ffn
RMSD bond length	0.003
RMSD bond angle	0.582
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.470	4.100
High resolution limit [Å]	3.800	3.800
Rmeas	0.319
Number of reflections	22724	4580
<I/σ(I)>	8.1	2.2
Completeness [%]	99.9	100
Redundancy	11.3	11.9
CC(1/2)	0.993	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	1.5 M ammonium sulfate, 20% glycerol, 100 mM Tris-HCl