6Q9O
HDM2 (17-111, WILDTYPE) COMPLEXED WITH COMPOUND 10 AT 1.21A; Structural states of Hdm2 and HdmX: X-ray elucidation of adaptations and binding interactions for different chemical compound classes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.929, 58.976, 39.880 |
| Unit cell angles | 90.00, 106.82, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.210 |
| R-factor | 0.1453 |
| Rwork | 0.145 |
| R-free | 0.15580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oq3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.250 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.069 | 0.262 |
| Number of reflections | 50939 | 5176 |
| <I/σ(I)> | 28.44 | 4.54 |
| Completeness [%] | 99.5 | 95.6 |
| Redundancy | 6.1 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 2.4M AmSO4, 0.1M NaCitrate, 0.2M NaCl, 0.1M TRIS |
