6Q8R

HEW Lysozyme under 2 kbar of argon

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.7712
Spacegroup name	P 43 21 2
Unit cell lengths	77.830, 77.830, 37.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.950 - 1.700
R-factor	0.15445
Rwork	0.153
R-free	0.18798
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5fst
RMSD bond length	0.010
RMSD bond angle	1.690
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	FFT
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.780
High resolution limit [Å]	1.700	1.700
Rmeas	0.083	0.472
Number of reflections	12830	930
<I/σ(I)>	19.03	2.4
Completeness [%]	94.4	58.3
Redundancy	18	8.5
CC(1/2)	0.999	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	298	10 % (W/V) NACL, 0.1 M ACETATE BUFFER PH 4.8. + 25 % (V/V) ETH-GLYCOL