6Q84

Crystal structure of RanGTP-Pdr6-eIF5A export complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9779
Spacegroup name	P 63
Unit cell lengths	139.540, 139.540, 346.570
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.173 - 3.700
R-factor	0.2273
Rwork	0.206
R-free	0.24710
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.173	3.832
High resolution limit [Å]	3.700	3.700
Rmeas	0.101	3.081
Rpim	0.030	0.953
Number of reflections	40583	4014
<I/σ(I)>	17.74	0.89
Completeness [%]	99.8	99.65
Redundancy	10.8	10
CC(1/2)	1.000	0.527

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	291	100 mM Sodium acetate pH 5.3 and 30% PEG 300