6Q83

Crystal structure of the biportin Pdr6 in complex with UBC9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9779
Spacegroup name	H 3 2
Unit cell lengths	198.260, 198.260, 289.630
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.565 - 4.530
R-factor	0.2597
Rwork	0.259
R-free	0.27080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2GJD and 6Q82
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.565	49.565	4.650
High resolution limit [Å]	4.530	20.260	4.530
Rmerge	0.168	0.028	2.492
Rmeas	0.178	0.030	2.638
Number of reflections	12937	165	892
<I/σ(I)>	11.16	60.66	0.92
Completeness [%]	99.6	94.8	95.7
Redundancy	10.072	9.109	9.225
CC(1/2)	0.999	0.999	0.578

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	293	Sodium cacodylate, magnesium chloride, ethanol