6Q78
The structure of GH26C from Muricauda sp. MAR_2010_75
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.119, 60.358, 148.995 |
| Unit cell angles | 90.00, 95.92, 90.00 |
Refinement procedure
| Resolution | 48.839 - 1.500 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.19060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vx6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.066 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 1.520 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.117 | |
| Rpim | 0.053 | 0.424 |
| Number of reflections | 132761 | 6137 |
| <I/σ(I)> | 9.5 | 2.2 |
| Completeness [%] | 99.5 | 93.6 |
| Redundancy | 6.7 | 6.4 |
| CC(1/2) | 0.995 | 0.668 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2 M Li2SO4, 0.1 M Bis-Tris:HCl pH 5.5, 24 % w/v PEG3350 |
