6Q6Z
Structure of the plant immune signaling node EDS1 (enhanced disease susceptibility 1) in complex with nanobody ENB21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976254 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 142.192, 142.192, 97.802 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 61.571 - 3.476 |
R-factor | 0.2214 |
Rwork | 0.218 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nfu |
RMSD bond length | 0.003 |
RMSD bond angle | 0.765 |
Data reduction software | XDS (XDS VERSION JAN 26, 2018, AUTOPROC VERSION JAN 26, 2018) |
Data scaling software | autoPROC (autoPROC (Version 1.0.5), AIMLESS (version 0.7.3), CCP4 7.0.065, STARANISO) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.586 | 3.646 |
High resolution limit [Å] | 3.473 | 3.473 |
Rmerge | 0.182 | |
Rmeas | 0.187 | |
Rpim | 0.043 | |
Number of reflections | 12634 | 634 |
<I/σ(I)> | 12.1 | |
Completeness [%] | 90.8 | 57.1 |
Redundancy | 19.5 | 20.1 |
CC(1/2) | 0.993 | 0.354 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | CRYSTALLIZATION CONDITIONS: RESERVOIR COMPOSITION (CONDITION A11 of MORPHEUS SCREEN): 20.0 % (V/V) GLYCEROL, 10.0 % (W/V) PEG 4000, 0.03 M MAGNESIUM CHLORIDE, 0.03 M CALCIUM CHLORIDE, 0.0609 M TRIS-BASE, 0.0391 M BICINE, PH 8.5; PROTEIN STOCK SOLUTION: 4.1 MG/ML PROTEIN, 50 MM SODIUM CHLORIDE, 1 % (V/V) GLYCEROL, 1 MM DTT, 50 MM HEPES, PH 8.0; DROP COMPOSITION: 150 NL PROTEIN STOCK SOLUTION PLUS 150 NL RESERVOIR SOLUTION; CRYO CONDITIONS: THE CRYSTALS WERE FLASH FROZEN DIRECTLY FROM THE EQUILIBRATED CRYSTALLIZATION DROPS. |