6Q6R
Recognition of different base tetrads by RHAU: X-ray crystal structure of G4 recognition motif bound to the 3-end tetrad of a DNA G-quadruplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-16 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.390, 42.250, 61.404 |
| Unit cell angles | 90.00, 99.30, 90.00 |
Refinement procedure
| Resolution | 60.600 - 1.500 |
| R-factor | 0.18832 |
| Rwork | 0.185 |
| R-free | 0.24481 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2lk7 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.947 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.600 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 45681 | 7212 |
| <I/σ(I)> | 8.68 | 1.62 |
| Completeness [%] | 99.3 | 98.3 |
| Redundancy | 3.827 | 3.62 |
| CC(1/2) | 0.998 | 0.816 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 55% methylpentanediol, 40 mM sodium cacodylate (pH 6.0), 80 mM potassium chloride and 12 mM spermine tetrahydrochloride |
