6Q5T

Crystal structure of Mycolicibacterium hassiacum glucosylglycerate hydrolase (MhGgH) - apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96863
Spacegroup name	P 62 2 2
Unit cell lengths	167.008, 167.008, 243.266
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.860 - 2.540
R-factor	0.1682
Rwork	0.166
R-free	0.20380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ohz
RMSD bond length	0.009
RMSD bond angle	1.058
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.860	2.630
High resolution limit [Å]	2.540	2.540
Rmerge	0.109	1.165
Rmeas	0.117	1.254
Rpim	0.043	0.459
Number of reflections	66272
<I/σ(I)>	11.9	1.8
Completeness [%]	99.8	99.7
Redundancy	7.2	7.3
CC(1/2)	0.998	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	ADA pH 6.5, ammonium sulfate